Chromogenic Substrates

<p>For the detection of beta-galactosidase activity</p>

<p>6-Chloro-3-indoxyl butyrate is a chemiluminescent substrate that is used in diagnostics and research. It has been shown to be an excellent ligand for the detection of DNA and protein. 6-Chloro-3-indoxyl butyrate can also be used as a chromogenic substrate for the detection of alkaline phosphatase, acid phosphatase, or beta-galactosidase. This product is also used in food testing, enzyme substrates, and fluorogenic substrates.</p>

<p>2-Nitrophenyl-1-thio-beta-D-galactopyranoside is a nitroaryl compound that can be synthesized from beta-D-galactopyranosides and nitric acid. It has a molecular conformation that is stabilized by hydrogen bonds, which allows it to form supramolecular assemblies with other molecules. The nitro group in this molecule is responsible for the formation of the nitro group in 1,2,4,5,-tetranitrobenzene.</p>

<p>5-Bromo-4-chloro-3-indolyl a-L-rhamnoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-L-rhamnosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-L-rhamnoside is used in bacterial rhamnose metabolism studies (e.g. E. coli).</p>

<p>4-Nitrophenyl 6-O-(alpha-D-glucopyranosyl)-beta-D-glucopyranoside is a chromogenic substrate for beta-glucosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 6-O-(alpha-D-glucopyranosyl)-beta-D-glucopyranoside is used in different applications such as the Cellulose metabolism research, biofuel production</p>

<p>This powerful chromogenic substrate is designed specifically for the assay and detection of a-L-fucosidase enzyme activity. With a unique combination of bromo, chloro, and indolyl moieties, it offers enhanced stability and promotes color development that is unparalleled in clarity and intensity. Ideal for biochemistry, molecular biology, and related life sciences applications, this substrate provides unmatched performance and reliability for all your a-L-fucosidase enzyme research needs.</p>

<p>4-Nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside is a chromogenic substrate for alpha-L-arabinofuranosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside is used in different applications such as the Plant biomass analysis, bacterial arabinan degradation assays</p>

<p>3-Indoxyl phosphate, p-toluidine salt is a fluorogenic substrate that can be used for the detection of β-galactosidase. 3-Indoxyl phosphate, p-toluidine salt is a highly active chromogenic substrate that can be used as a diagnostic tool in the food industry to determine whether or not milk has been adulterated with animal milk. This product is also used in environmental testing to detect bacteria and fungi. 3-Indoxyl phosphate, p-toluidine salt is a high purity, high quality conjugate that can be used for the detection of β-galactosidase.</p>

<p>5-Bromo-4-chloro-3-indolyl β-L-fucopyranoside (X-Fuc) is a chromogenic substrate specifically designed for the identification and detection of fucosidase enzyme activity. Upon cleavage by fucosidase, it generates a blue-colored 5-Bromo-4-chloro-3-hydroxy-indole precipitate, which can be easily observed under a microscope or spectrophotometer. This feature makes X-Fuc an invaluable tool across various applications, such as biochemical analysis, histochemical staining, cell biology, and enzyme kinetic assays. By providing a fast, sensitive, and reliable readout for fucosidase activity, 5-Bromo-4-chloro-3-indolyl β-L-fucopyranoside facilitates essential research in the understanding of biological processes and the development of therapeutic interventions.</p>

<p>Chlorophenol red beta-D-galactopyranoside sodium salt is a water soluble, colorless salt that is used as a substrate for the reduction of contaminants in soil and water. It can be used to measure the activity of microorganisms in the environment and has been shown to inhibit growth of certain bacteria, such as methylotrophic bacteria. Chlorophenol red beta-D-galactopyranoside sodium salt is also used in biological treatment systems. This product can be mixed with other substances in order to regulate the microbial population and reduce nutrients.</p>

<p>Substrate for hydrolases</p>

<p>5-Bromo-6-chloro-3-indolyl-2-acetamido-2-deoxy-a-D-glucopyranoside is a chemiluminescent substrate that is used as an indicator in diagnostic tests. This compound reacts with an oxidizing agent, such as hydrogen peroxide, to generate light. It is also used as a chromogenic substrate for the detection of bioluminescence and it can be applied in culture media or food testing. 5Bromo-6Chloro-3Indolyl2Acetamido2DeoxyA1DGlucopyranoside has a CAS number, which means that it meets the standards set by the Chemical Abstracts Service. It also has a high purity and quality, making it suitable for use in laboratories.</p>

<p>Alternative chromogenic substrate for alpha-D-galactosidase</p>

<p>Chromogenic substrate yielding a soluble yellow chromophor for the colorimetric assay of α-L-fucosidase, especially in human serum.</p>

<p>Chromogenic substrate for β-D-Cellobiosidase yielding a yellow solution upon cleavage.</p>

<p>Chromogenic substrate for α-galactosidase yielding a salmon-colored precipitate.</p>

<p>5-Bromo-6-chloro-3-indoxyl butyrate is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in lipid digestion assays.</p>

<p>Substrate for beta-galactosidase</p>

<p>Also known as Lapis-caprylate; targeting esterase with C8 activity; Blue color</p>

<p>4-Nitrophenyl 2-O-(beta-D-glucopyranosyl)-beta-D-glucopyranoside is a chromogenic substrate for beta-glucosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 2-O-(beta-D-glucopyranosyl)-beta-D-glucopyranoside is used in different applications such as the Cellulose degradation studies, biofuel research</p>

<p>Chromogenic beta-Glucosidase substrate yielding a yellow solution upon cleavage.</p>

<p>Chromogenic substrate for aryl-sulfatase. Yields a blue precipitate upon cleavage.</p>

<p>6-Bromo-2-naphthyl beta-D-glucuronide is a substrate for beta-glucuronidase, an enzyme involved in detoxification and bacterial metabolism. Upon enzymatic hydrolysis, it releases 6-bromo-2-naphthol, which forms a detectable dye when reacted with diazonium salts. This compound is widely used in microbiology to detecting E. coli in water testing and histochemical staining. 6-Bromo-2-naphthyl beta-D-glucuronide is also been used in cancer research studies. It has been found that some tumors are related to a high activity of beta-glucuronidase, responsible to reactivate the carcinogens and promote tumor progression, developing a drug resistance to chemotherapy.</p>

<p>4-Nitrophenyl b-D-thioglucopyranoside is a highly sensitive chromogenic substrate designed for the colorimetric detection and quantification of thioglucosidase enzymes. Upon enzymatic cleavage, it releases a highly absorbing 4-nitrophenyl moiety, generating a yellow color that can be readily measured spectrophotometrically. This substrate is widely utilized in the study of enzyme kinetics, substrate specificity, and inhibitor screening for thioglucosidases involved in various metabolic pathways and biological processes.</p>

<p>Chromogenic substrate for β-D-galactosidase yielding a precipitate. Mostly used in histochemistry.</p>

<p>1-Naphthyl β-D-mannopyranoside is a chromogenic (also fluorogenic) substrate. After cleavage, 1-naphthol is released and is used to quantify beta-mannosidase activity.</p>

<p>N-(3-Sulfopropyl)-3,3',5,5'-tetramethylbenzidine (TMB) sodium salt is a highly sensitive chromogenic substrate used for the detection and quantification of peroxidase enzyme activity. This water-soluble substrate undergoes a color change from colorless to blue upon oxidation by peroxidase enzymes in the presence of hydrogen peroxide. The reaction can be stopped by adding an acid, resulting in a yellow color that can be measured spectrophotometrically. TMB sodium salt is widely used in various applications, including enzyme-linked immunosorbent assays (ELISA), immunohistochemistry, and other diagnostic tests, due to its high sensitivity, low background signal, and excellent stability.</p>

<p>Naphthol AS-BI sulphate potassium salt is a modified naphtol AS substrate used for alkaline and acid phosphatase.</p>

<p>Chromogenic substrate for alkaline and acid phosphatase</p>

<p>3,3'-Diaminobenzidine (DAB) is an organic compound derived from benzidine. DAB is water-soluble as the tetrahydrochloride salt and is used in immunohistochemical staining of nucleic acids and proteins. Within research in Alzheimer's disease, Aβ protein amyloid plaques are targeted by a primary antibody, and subsequently by a secondary antibody, which is conjugated with a peroxidase enzyme. This will bind DAB as a substrate and oxidize it, producing an easily observable brown colour. 3,3’-Diaminobenzidine tetrahydrochloride hydrate is also available as the tetrahydrochloride salt and as the free base.</p>

<p>5-Iodo-3-indolyl-b-D-galactopyranoside-6-sulfate sodium salt is a high purity, water soluble fluorogenic substrate for the detection of beta-galactosidase activity. This product is used in food testing, environmental testing, and diagnostics. 5-Iodo-3-indolyl-b-D-galactopyranoside (5IIAG) is a beta galactosidase substrate that provides a convenient method for determining beta galactosidase activity. This product is also used as an indicator to measure the rate of hydrolysis of ester bonds in other compounds that contain acid functional groups such as glycerides and oils.</p>

<p>4-Nitrophenyl 2-acetamido-3-O-[2-O-a-L-fucopyranosyl-b-D-galactopyranosyl)-2-deoxyb-D-galactopyranoside is a water soluble, fluorescent probe that can be used for detecting the presence of β-(1→4) -linked galactosaminoglycans. The dye is a substrate for enzymes such as α--mannosidase, β--glucuronidase and β--galactosidase. The dye is also an excellent ligand for lectins such as concanavalin A and wheat germ agglutinin. This product is a diagnostic reagent that has been shown to have high purity and quality. It has CAS No. 93496-53-8 and can be used in food testing, bioluminescence, environmental testing, or high purity applications.</p>

<p>Aldol 458 nonanoate solution (0.75 M) is a substrate for enzymes and ligands, which is used in various applications such as food testing, staining, fluorogenic substrate, environmental testing, chromogenic substrate and conjugate. Aldol 458 nonanoate solution (0.75 M) has high purity and is highly stable at pH 2-12. This product can be used as a chemiluminescence or bioluminescence substrate. The CAS number for this product is 2484873-15-4.</p>

<p>1-Naphthyl a-D-galactopyranoside is a chromogenic substrate use to detect alpha-galactosidase activity.</p>

<p>Ostazin Brilliant Red H-3B is a water soluble, non-toxic, and non-hazardous substrate for enzymes that catalyze reactions involving the cleavage of β-D-glucosides. It is used as a chromogenic or fluorogenic substrate in diagnostic tests. Ostazin Brilliant Red H-3B can also be used in bioluminescence reaction with luciferase to measure ATP levels in living cells.</p>

<p>Aldol® 514 inositol-phosphate is a chromogenic enzyme substrate used for detection of phosphatidylinositol specific phospholipase C (PI-PLC, EC 4.6.1.13). The colorless enzyme substrate is cleaved by PI-PLC positive microorganisms, yielding a red-orange color. The substrate thus enables the detection and identification of various bacteria, such as, pathogenic strains of Listeria, Clostridium, Bacillus, and Staphylococcus. Aldol® 514 inositol-phosphate is suitable for use under aerobic and anaerobic conditions.</p>

<p>Aldol® 515 phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatase activity. The colorless enzyme substrate is cleaved by acid and alkaline phosphatases secreted by bacteria in liquid or solid media, yielding intense red color and red fluorescence. Aldol® 515 phosphate can also be used in Western blots and other immunoassay applications with phosphatase-coupled secondary antibodies. Aldol® 515 phosphate can be used under aerobic and anaerobic conditions.</p>

<p>Chromogenic substrate for α-D-Fucosidase yielding a blue precipitate.</p>

<p>This product is a chromogenic substrate that is used in the detection of alkaline phosphatase and other enzymes. It is also used in food testing, staining, and diagnostics. This product has high purity with a CAS number of 125328-84-9.</p>

<p>N-Acetyl-5-bromo-3-indoxyl-2,3,4-tri-O-acetyl-beta-D-glucuronic acid methyl ester is a chromogenic substrate that can be used for the detection of bile acids and bilirubin in food testing. It is a conjugate of an acetylated 2,3,4 triacetyl glucuronic acid methyl ester with 5 bromo 3 indoxyl. This product has high purity and high quality.</p>

<p>Chromogenic substrate for β-D-Cellobiosidase yielding a blue precipitate.</p>

<p>4-Nitrophenyl myristate (NphOMy) is a chromogenic enzyme substrate used as a diagnosis tool for detection of β-lactamase hydrolysis activities. In the presence of certain drugs, 4-nitrophenyl myristate HSA-Tyr411-catalyzed hydrolysis seems to be inhibited.</p>

<p>Aldol® 535 myo-inositol-1-phosphate, ammonium salt is a chromogenic substrate for the enzyme, phosphoglucomutase. This product is used for the detection of phosphoglucomutase by its ability to react with this enzyme and produce a blue or green color. Aldol® 535 myo-inositol-1-phosphate, ammonium salt is also used as a ligand in fluorescence resonance energy transfer (FRET) experiments. It has an EC number of CAS No. 2484872-80-0 and a molecular weight of 345.05 g/mol.</p>

<p>Aldol® 470 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-galactosidase activity. The colorless enzyme substrate is cleaved by lactose-utilizing bacteria grown in liquid or solid media, yielding yellow coloration and green fluorescence. Aldol® 470 beta-D-galactopyranoside can also be used in beta-galactosidase gene reporter assays or immunoassay applications (ELISA). Aldol® 470 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>8-Hydroxyquinoline b-D-glucopyranoside (8-OHG-glu) is a chromogenic enzyme substrate commonly used to detect the presence of beta-glucosidase in biological samples. When hydrolyzed by beta-glucosidase, 8-OHG-glu produces a yellow-orange color, indicating the presence of the enzyme. This substrate is particularly useful for measuring beta-glucosidase activity in soil and plant samples.</p>

<p>Aldol 470 acetate is a chromogenic substrate for carboxylesterases yielding a yellow precipitate. Furthermore it shows some fluorescence(excitation at 320 nm to 490 nm). In contrast to classical indoxylsubstrates, Aldol substrates work not only under aerobic but also under anaerobic conditions.</p>

<p>Aldol® 515 N-glutaryl-L-phenylalanine amide is a chromogenic and fluorogenic enzyme substrate used for detection of L-glutamate-phenylalanine (GluPhe)-specific peptidase activity. The colorless enzyme substrate is cleaved the GluPhe-specific peptidase, yielding development of orange-red coloration. In addition, red fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 515 N-glutaryl-L-phenylalanine amide can be used in enzyme assays and live bacterial cultures. Aldol® 515 N-glutaryl-L-phenylalanine amide is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.</p>

<p>Aldol® 515 caprylate is a chromogenic and fluorogenic enzyme substrate used for detection of lipase activity. The colorless enzyme substrate is cleaved by C8 esterases, yielding development of red coloration. In addition, red fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 515 caprylate can be used in enzyme assays and live bacterial cultures. Aldol® 515 caprylate is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.</p>

<p>Naphthol AS-OL is a widely used chromogenic enzyme substrate that forms a colored product upon reaction with specific enzymes. It is commonly employed in histochemical and cytochemical staining techniques to visualize enzyme activity in cells and tissues. Naphthol AS-OL is particularly useful for detecting the presence of enzymes such as alkaline phosphatase, acid phosphatase, and esterases. The resulting colored product can be easily observed under a microscope, allowing researchers to study the distribution and localization of these enzymes in various biological samples.</p>

<p>5-Iodo-3-indoxyl-beta-D-galactopyranoside is a chromogenic substrate for beta-galactosidase.</p>