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Chromogenic Substrates

Chromogenic Substrates

Chromogenic substrates are compounds that produce a visible color change when acted upon by specific enzymes. These substrates are widely used in diagnostic assays, biochemical research, and enzyme-linked immunosorbent assays (ELISAs) to detect and quantify enzyme activity. The color change allows for easy and rapid visual identification of enzyme reactions, making chromogenic substrates essential tools in laboratory analysis. At CymitQuimica, we provide a diverse selection of high-quality chromogenic substrates to support your research and diagnostic applications.

Found 712 products of "Chromogenic Substrates"

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  • Phenolphthalein b-D-glucuronide sodium salt monohydrate

    CAS:
    Monohydrate; beta-glucuronidase substrate; yellow/pink color Beta-glucuronidase activity can be measured in a colorimetric assay with phenolphthalein beta-D-glucuronide as substrate. Starting assay conditions can include 30 µg of enzyme-containing material, 0.6 mM phenolphthalein beta-D-glucuronide in 100 mM sodium phosphate buffer in a total volume of 100 µL and monitoring absorbance changes at 540 nm. Temperature incubation can be used to promote the enzymatic activity (Galindo, 2012).
    Formula:C26H23NaO11
    Purity:Min. 95%
    Color and Shape:Off-White Powder
    Molecular weight:534.45 g/mol

    Ref: 3D-EP06598

    25mg
    225.00€
    50mg
    389.00€
    100mg
    574.00€
    250mg
    1,070.00€
    500mg
    1,675.00€
  • Ostazin brilliant red H-3B galactomannan


    Ostazin Brilliant Red H-3B Galactomannan is a chemiluminescent substrate used for the detection of β-1,4-galactomannans. It reacts with the glucose residues on the mannan backbone to produce a red fluorescent product. This product can be detected by spectrophotometry at an excitation wavelength of 395 nm and emission wavelength of 510 nm. The Ostazin Brilliant Red H-3B Galactomannan substrate is useful in detecting Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumoniae, Group A streptococci, Group B streptococci, Group D streptococci, Group G streptococci, and Group C streptococci.

    Purity:Min. 95%

    Ref: 3D-EO164897

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  • 4-Nitrophenyl-beta-D-glucopyranoside

    CAS:
    Chromogenic β-glucosidase substrate yielding a yellow solution upon cleavage.
    Formula:C12H15NO8
    Purity:Min. 99.0 Area-%
    Molecular weight:301.25 g/mol

    Ref: 3D-N-4400

    5g
    588.00€
    10g
    928.00€
    25g
    1,968.00€
    50g
    3,408.00€
    100g
    5,964.00€
  • 3-Indolyl 2-acetamido-2-deoxy-b-D-glucopyranoside

    CAS:
    3-Indolyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a substrate used for the detection of the enzyme N-acetylglucosaminidase (NAG). The enzyme hydrolyzes the NAG conjugate, releasing indole that can be detected through a colorimetric assay. This method is commonly used in clinical chemistry to evaluate kidney function, as NAG is excreted in urine and increased levels may indicate renal injury or disease. The indole NAG assay is also used in microbiology to detect the presence of NAG-producing bacteria.
    Purity:Min. 95%
    Molecular weight:336.34 g/mol

    Ref: 3D-EI03306

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  • 6-Nitro-3-indolyl-b-D-galactopyranoside


    6-Nitro-3-indolyl-b-D-galactopyranoside is a chemiluminescent substrate that reacts with horseradish peroxidase to produce light. 6NIGP is used in the diagnostic field as a fluorogenic or chromogenic substrate for enzymes such as alkaline phosphatase, and as a staining agent for cells, tissues, and other biological materials. 6NIGP has been used in the detection of bacteria, fungi, parasites, and viruses. It is also used in food testing as an enzyme substrate. 6NIGP is purified by recrystallization from water or acetone and can be stored at −20°C.
    Purity:Min. 95%

    Ref: 3D-EN172467

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  • Naphthol AS-BI N-acetyl-b-D-galactosaminide

    CAS:
    Modified naphtol AS substrate used in histochemical and cytochemical staining
    Formula:C26H27BrN2O8
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:575.41 g/mol

    Ref: 3D-EN09607

    1g
    538.00€
    50mg
    135.00€
    100mg
    189.00€
    250mg
    268.00€
    500mg
    403.00€
  • 2-Naphthyl caprylate

    CAS:

    Chromogenic substrate for carboxylesterase and lipase. 2-naphthol is released upon hydrolyzation. By simultaneous coupling with a diazonium salt, the corresponding azo-dye is formed. Naphthols can also be detected by fluorescence analysis. Used in an colorimetric assay for the extracellular lipase of Pseudomonas fluorescens B52.

    Formula:C18H22O2
    Purity:Min. 98.0 Area-%
    Molecular weight:270.37 g/mol

    Ref: 3D-N-2220

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  • 5-Bromo-3-indoxyl caprylate

    CAS:

    5-Bromo-3-indoxyl caprylate is a fluorogenic substrate that can be used for the detection of β-galactosidase. It is an enzyme substrate that can be used to measure β-galactosidase activity in cell culture media. It has a high purity and high quality. 5-Bromo-3-indoxyl caprylate is also used as a chromogenic substrate for the detection of β-galactosidase. This product has a CAS number of 133950-69-3.

    Formula:C16H20BrNO2
    Purity:Min. 99 Area-%
    Molecular weight:338.24 g/mol

    Ref: 3D-B-8850

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  • 3,4-Cyclohexenoesculetine phosphate disodium


    Chromogenic substrate for phosphatase
    Purity:Min. 95%

    Ref: 3D-EC183616

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  • 4-Chloro-1-naphthol

    CAS:
    Substrate for horseradish peroxidase (HRP). HRP catalyzes oxidation of 4-chloro-1-naphthol in the presence of peroxide to the blue insoluble product 4-chloro-1-naphthon. It is used for both blotting and immunohistochemical staining but is less sensitive than other precipitating HRP substrates.
    Formula:C10H7ClO
    Purity:Min. 97.0 Area-%
    Molecular weight:178.62 g/mol

    Ref: 3D-C-5180

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  • 5-Nitro-3-indolyl-b-D-galactopyranoside


    5-Nitro-3-indolyl-b-D-galactopyranoside is a substrate for a number of enzymes, including beta-galactosidase, beta-glucuronidase, and beta-mannosidase. It is used as an enzyme substrate in diagnostics to measure the activity of these enzymes. 5N3IG was also used to study the effects of conjugation on enzyme activity. 5N3IG is available in high purity and high quality at CAS No.

    Purity:Min. 95%

    Ref: 3D-EN172466

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  • 1-Naphthyl β-D-glucopyranoside

    CAS:

    1-Naphthyl β-D-glucopyranoside can be used as a chromogenic substrate for beta-glucosidases. It can also be used as a fluorogenic substrate to study carbohydrate metabolism and in screening enzyme inhitibitors in pharmacologic research.

    Formula:C16H18O6
    Purity:Min. 95%
    Color and Shape:White Off-White Powder
    Molecular weight:306.31 g/mol

    Ref: 3D-EN05127

    5g
    242.00€
    10g
    408.00€
    25g
    784.00€
    50g
    1,202.00€
  • 2-Naphthyl-Beta-D-Glucuronide Sodium Salt

    CAS:
    2-Naphthyl-beta-D-glucuronide sodium salt is an enzyme substrate to detect beta-glucuronidase. The enzyme cleaves the glycosidic bond in 2-naphthyl-beta-D-glucuronide to release 2-naphtol and glucuronic acid. Beta-glucuronidase activity assays are important in many fields such as cancer research, as the enzyme is expressed in some tumors and its activity can be targeted for cancer therapy. The enzyme has also an important role in drug metabolism as it recognizes and binds to drug glucuronide conjugate (as 2-naphthyl-beta-D-glucuronide) facilitating its hydrolysis to release the active part. 2-Naphthyl-beta-D-glucuronide sodium salt has a higher solubility as it is in its salt form.
    Formula:C16H15NaO7
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:342.28 g/mol

    Ref: 3D-EN03259

    5mg
    281.00€
    10mg
    402.00€
    25mg
    705.00€
    50mg
    1,008.00€
    100mg
    1,594.00€
  • Aldol® 458 beta-D-galactopyranoside, Biosynth Patent: EP 2427431 and US 8940909

    CAS:
    Aldol® 458 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-galactosidase activity. The colorless enzyme substrate is cleaved by lactose utilizing bacteria grown in liquid or solid media, yielding yellow coloration and green fluorescence. Aldol® 458 beta-D-galactopyranoside can also be used in beta-galactosidase gene reporter assays or immunoassay applications (ELISA). Aldol® 458 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.
    Formula:C22H23NO8
    Purity:Min. 95 Area-%
    Color and Shape:Powder
    Molecular weight:429.42 g/mol

    Ref: 3D-EA175345

    1g
    449.00€
    5g
    1,202.00€
    10g
    1,603.00€
    2500mg
    850.00€
  • 3-Nitrophenyl β-D-glucopyranoside

    CAS:
    3-Nitrophenyl beta-D-glucopyranoside is a chromogenic enzyme substrate commonly used to assay for beta-glucosidase activity. Upon hydrolysis by the enzyme, it produces a yellow product that can be easily detected by spectrophotometry. This substrate is often used in the field of carbohydrate chemistry and biochemistry to study enzyme kinetics and inhibition in both in vitro and in vivo systems.
    Formula:C12H15NO8
    Purity:Min. 95%
    Color and Shape:White Yellow Powder
    Molecular weight:301.25 g/mol

    Ref: 3D-EN06883

    5g
    350.00€
    10g
    478.00€
    25g
    935.00€
  • 6-Chloro-3-indoxyl nonanoate

    CAS:

    6-Chloro-3-indoxyl nonanoate is a fluorescent substrate that is used in the detection of beta-galactosidase activity. It has been used to detect the enzyme levels in various culture media and as a high quality, food testing, and environmental testing. The product is also used as a ligand for enzyme inhibition studies. 6-Chloro-3-indoxyl nonanoate has shown to be an excellent fluoroquinolone substrate for chemiluminescence assays. This product is CAS No. 133950-72-8 and is of high purity and high quality.

    Purity:Min. 95%
    Molecular weight:307.81 g/mol

    Ref: 3D-EC32009

    1g
    3,122.00€
  • 4-Nitrophenyl laurate

    CAS:
    4-Nitrophenyl laurate is a chromogenic substrate for lipase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl laurate is used in different applications such as the Lipase activity screening, clinical diagnostics
    Formula:C18H27NO4
    Purity:Min. 95%
    Color and Shape:White Powder
    Molecular weight:321.41 g/mol

    Ref: 3D-EN15367

    50g
    354.00€
    100g
    495.00€
    250g
    937.00€
    500g
    1,344.00€
  • 4-Nitrophenyl sulfate potassium salt

    CAS:
    4-Nitrophenyl sulfate potassium salt is a chromogenic substrate for arylsulfatase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl sulfate potassium salt is used in different applications such as the Metachromatic leukodystrophy diagnosis, sulfatase activity assays
    Formula:C6H4KNO6S
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:257.26 g/mol

    Ref: 3D-EN27113

    1g
    263.00€
    2g
    462.00€
    5g
    854.00€
    10g
    1,499.00€
    25g
    2,343.00€
  • 8-Hydroxyquinoline b-D-galactopyranoside

    CAS:
    Chromogenic substrate targeting the beta-galactosidase enzyme
    Formula:C15H17NO6
    Purity:Min. 98 Area-%
    Color and Shape:Powder
    Molecular weight:307.3 g/mol

    Ref: 3D-EH05121

    2g
    260.00€
    5g
    416.00€
    10g
    641.00€
  • Aldol® 470 L-pyroglutamic acid amide, Biosynth Patent: EP 2427431 and US 8940909

    Controlled Product
    CAS:
    Aldol® 470 L-pyroglutamic acid amide is a chromogenic and fluorogenic enzyme substrate used for detection of pyrrolidonyl peptidase (PYRase) activity. The colorless enzyme substrate is cleaved by PYRase-producing bacteria grown in liquid or solid media, yielding orange color. In addition, green fluorescence is generated when a suitable matrix is present, such as, the surface of cellulose acetate filters, polypropylene tubes or microplates, and polyethylene vials. Aldol® 470 L-pyroglutamic acid amide is suitable for use under aerobic and anaerobic conditions.
    Formula:C28H25N3O5
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:483.52 g/mol

    Ref: 3D-EA175364

    1g
    494.00€
    5g
    1,403.00€
    10g
    1,952.00€
    2500mg
    957.00€
  • 5-Bromo-6-chloro-3-indoxyl choline phosphate

    CAS:
    5-Bromo-6-chloro-3-indoxyl choline phosphate is a chemiluminescent substrate for the detection of bacterial activity. It reacts with an enzyme, luciferase, which is present in a variety of living cells. The reaction produces light. This product has been tested for environmental and food safety by the manufacturer. 5-Bromo-6-chloro-3-indoxyl choline phosphate is used as a diagnostic tool to determine the presence of bacteria in humans, animals, and plants. It can be used to measure levels of bacteria in culture media, water samples, and food products. 5-Bromo-6-chloro-3-indoxyl choline phosphate can also be used as a substrate for enzymes such as phosphatases or esterases that catalyze reactions with substrates with brominated or chlorinated groups.
    Formula:C13H17BrClN2O4P
    Purity:Min. 95%
    Molecular weight:411.63 g/mol

    Ref: 3D-EA175405

    50mg
    305.00€
    100mg
    395.00€
    250mg
    699.00€
  • 4-Nitrophenyl 2-acetamido-6-O-(2-acetamido-2-deoxy-b-D-glucopyranosyl)-2-deoxy-a-D-galactopyranoside

    CAS:
    4-Nitrophenyl 2-acetamido-6-O-(2-acetamido-2-deoxy-b-D-glucopyranosyl)-2-deoxy-a-D-galactopyranoside is a chromogenic pNP glycoside substrate designed for the detection and assay of specific enzymes, particularly glycosidases. Upon enzymatic hydrolysis, this substrate releases a quantifiable 4-nitrophenol moiety enabling straightforward colorimetric measurement. This pNP-linked substrate is versatile and can be efficiently employed in enzyme activity assays, screening enzyme inhibitors, and assessing enzyme mechanisms, enhancing research and development processes in the field of glycobiology.
    Purity:Min. 95%
    Color and Shape:White Powder
    Molecular weight:545.49 g/mol

    Ref: 3D-EN06448

    1mg
    663.00€
    2mg
    1,111.00€
    5mg
    2,253.00€
    10mg
    4,237.00€
    500µg
    443.00€
  • 5-Bromo-3-indolyl b-D-glucopyranoside

    CAS:
    5-bromo-3-indolyl-b-d-glucopyranoside, also known as 5BIG, is an enzyme substrate for beta-glucosidase. It is used in bacterial assays for the identifcation of Gram-positive bacteria such as Staphylococcus aureus and Bacillus subtilis. After cleavege, it gives an intense blue color.
    Formula:C14H16BrNO6
    Purity:Min. 98 Area-%
    Color and Shape:Powder
    Molecular weight:374.18 g/mol

    Ref: 3D-EB06829

    1g
    1,184.00€
    50mg
    338.00€
    100mg
    422.00€
    200mg
    715.00€
    500mg
    894.00€
  • N-Benzoyl-DL-arginine 4-nitroanilide hydrochloride

    CAS:

    N-α-benzoyl-DL-arginine-4-nitroanilide hydrochloride is commonly used as a substrate in various enzymatic assays, particularly for studying proteases like trypsin and chymotrypsin. It serves as a synthetic substrate to measure the activity of these enzymes. For instance, it has been used to determine trypsin activity in different organisms and tissues. The substrate has also been employed in studying the enzymatic properties of various proteases, including those found in latex, snake venoms,  and plant seeds. Additionally, it has been utilized to assess the effects of different compounds on trypsin activity, such as condensed tannins and wine.

    Formula:C19H22N6O4•HCl
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:434.88 g/mol

    Ref: 3D-EB32011

    2g
    213.00€
    5g
    355.00€
    10g
    527.00€
    25g
    891.00€
    50g
    1,428.00€
  • Aldol® 467 N-acetyl-beta-D-glucosaminide, Biosynth Patent: EP 2427431 and US 8940909

    CAS:
    Aldol® 467 N-acetyl-beta-D-glucosaminide is a chromogenic and fluorogenic enzyme substrate used for detection of ?-N-acetyl-glucosaminidase activity. The colorless enzyme substrate is cleaved in the presence of ?-N-acetyl-glucosaminidase yielding development of a yellow product. In addition, green fluorescence is generated when a suitable matrix or Aldol® 355 fluorescence enhancer is present. Aldol® 467 N-acetyl-beta-D-glucosaminide amide can be used in enzymatic assays or in live bacterial cultures. Aldol® 467 N-acetyl-beta-D-glucosaminide is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.
    Formula:C31H31ClN2O9
    Purity:Min. 92.0 Area-%
    Molecular weight:611.04 g/mol

    Ref: 3D-A-4671_P00

    1g
    382.00€
    5g
    966.00€
    10g
    1,214.00€
    2500mg
    680.00€
  • Aldol® 470 N-tosyl-L-alanine ester, Biosynth Patent: EP 2427431 and US 8940909

    Controlled Product
    CAS:
    The Aldol® 470 N-tosyl-L-alanine ester is a high purity, high quality fluorogenic substrate for the enzyme alkaline phosphatase. It is a conjugate of the tosyl group with L-alanine and has been used as a fluorogenic substrate in various food testing applications. The Aldol® 470 N-tosyl-L-alanine ester also has several other uses including in diagnostic methods, culture media, and enzyme substrates.
    Formula:C33H30N2O7S
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:598.67 g/mol

    Ref: 3D-EA175357

    1g
    494.00€
    5g
    1,403.00€
    10g
    1,952.00€
    2500mg
    957.00€
  • 4-Nitrophenyl 5-O-trans-feruloyl-a-L-arabinofuranoside

    CAS:

    4-Nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside is a chromogenic substrate for alpha-L-arabinofuranosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside is used in different applications such as the Plant cell wall degradation assays, bacterial enzyme profiling

    Formula:C21H21NO10
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:447.4 g/mol

    Ref: 3D-EN03543

    5mg
    274.00€
    10mg
    363.00€
    25mg
    606.00€
    50mg
    892.00€
    100mg
    1,322.00€
  • 4-Nitrophenyl caprylate

    CAS:
    Chromogenic substrate targeting lipase, C8 esterase
    Formula:C14H19NO4
    Purity:Min. 95 Area-%
    Color and Shape:Slightly Brown Yellow Clear Liquid
    Molecular weight:265.31 g/mol

    Ref: 3D-EN15366

    10g
    270.00€
    25g
    412.00€
    50g
    564.00€
    100g
    980.00€
    250g
    2,045.00€
  • Crimson-Gal


    Crimson-Gal (Crimson-beta-D-galactopyranoside) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields a crimson colored precipitate. Crimson-Gal can also be used as detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach. Read more in the Application Notes.
    Molecular weight:360.96 g/mol

    Ref: 3D-C-8570

    1g
    348.00€
  • N-Methyl-3-indolyl a-D-arabinopyranoside


    3-indolyl-N-methyl a-D-arabinopyranoside is a fluorogenic substrate for the detection of beta-galactosidase. It also has been used as a chromogenic substrate for the detection of beta-glucuronidase. This product can be conjugated to an enzyme and used in enzyme substrates for the detection of beta-galactosidase, beta-glucuronidase, and other enzymes. 3-indolyl-N-methyl a-D-arabinopyranoside is also commonly used in culture media to detect bacteria and yeast.
    Purity:Min. 95%
    Molecular weight:279.29 g/mol

    Ref: 3D-EM03253

    1g
    2,838.00€
  • 6-Chloro-1H-indol-3-yl b-D-mannopyranoside

    CAS:
    6-Chloro-1H-indol-3-yl b-D-mannopyranoside is an enzyme substrate that is used in the fluorogenic and chromogenic assays for detecting indoleamines. This compound has a conjugate, which is a fluorescent molecule that can be attached to an antibody to target specific proteins or cells. 6-Chloro-1H-indol-3-yl b-D-mannopyranoside is used as a diagnostic agent in the detection of bacterial and fungal infections. It is also used as an indicator of cell growth in culture media.
    Purity:Min. 95%
    Molecular weight:329.73 g/mol

    Ref: 3D-EC15884

    1g
    2,838.00€
  • Aldol® 515 palmitate, Biosynth Patent: EP 2427431 and US 8940909

    CAS:

    Aldol® 515 palmitate is a high quality, non-toxic and water soluble chromogenic substrate for enzymes. It is used to detect the presence of lipase activity due to yielding a red color.

    Formula:C39H50N2O3
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:594.83 g/mol

    Ref: 3D-EA175361

    1g
    674.00€
    5g
    1,914.00€
    10g
    2,664.00€
    2500mg
    1,231.00€
  • Aldol® 450 acetate, Biosynth Patent: EP 2427431 and US 8940909

    CAS:

    Aldol® 450 acetate is an enzyme substrate that is used in the detection of aldolase activity. Aldol® 450 acetate has been shown to be a useful alternative to the traditional bioluminescence and chemiluminescence methods for detecting aldolase activity. It has also been shown to be effective as a fluorogenic substrate for detecting aldolase activity in food products. Aldol® 450 acetate can be conjugated with other compounds, such as ligands and dyes, to enable it to detect different enzymes.
    Aldol® 450 acetate has an absorption maximum at 450 nm and fluoresces blue-green under longwave UV light excitation. It is soluble in water and exhibits excellent stability at high temperatures (up to 50°C) and pH (1-14).

    Formula:C27H19NO3
    Purity:Min. 95.0 Area-%
    Molecular weight:405.44 g/mol

    Ref: 3D-A-4728_P00

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  • Aldol® 515 propionate, Biosynth Patent: EP 2427431 and US 8940909

    CAS:
    Aldol® 515 propionate is a chromogenic substrate that is used as a reagent in food testing, diagnostics, and environmental testing. It reacts with an enzyme to form a fluorescent product that can be detected with a spectrophotometer. The substrate has been shown to have high purity and high quality.
    Formula:C26H24N2O3
    Purity:Min. 95.0 Area-%
    Molecular weight:412.48 g/mol

    Ref: 3D-A-4736_P00

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  • Aldol® 515 β-D-xylopyranoside, Biosynth Patent: EP 2427431 and US 8940909

    CAS:

    Aldol® 515 beta-D-xylopyranoside is a chromogenic substrate that is used in food testing. It is an alternative to the more common glucose and sucrose, which are not suitable for some types of assays. Aldol® 515 beta-D-xylopyranoside is also used in diagnostics as a ligand for fluorescent antibody staining and chemiluminescence assays. It has been shown to increase the sensitivity of these tests by up to 10 times. The substrate has been tested extensively and is safe for use with a wide range of bacteria including E. coli, S. typhimurium, M. tuberculosis, and C. albicans at concentrations up to 100 mM without any inhibitory effects on growth or viability.
    END>.

    Formula:C28H34N2O6
    Purity:Min. 95.0 Area-%
    Molecular weight:494.59 g/mol

    Ref: 3D-A-4754_P00

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  • Aldol® 495 phosphate, disodium salt, Biosynth Patent: EP 2427431 and US 8940909

    CAS:
    Aldol® 495 phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatase activity. The colorless enzyme substrate is cleaved by acid and alkaline phosphatases secreted by bacteria in liquid or solid media, yielding orange color and green fluorescence. Aldol® 495 phosphate can also be used in Western blots and other immunoassay applications with phosphatase-coupled secondary antibodies. Aldol® 495 phosphate can be used under aerobic and anaerobic conditions.
    Formula:C20H14ClN2Na2O5P
    Purity:Min. 98.0 Area-%
    Molecular weight:474.74 g/mol

    Ref: 3D-A-4704_P00

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  • 4-Nitrophenyl 3-O-(2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl)-b-D-glucopyranoside


    4-Nitrophenyl 3-O-(2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl)-b-D-glucopyranoside is a chromogenic substrate that is used to test for the presence of an enzyme. It reacts with the enzyme to produce a light signal that can be detected by a spectrophotometer. The CAS number for this compound is 832476-41-8. This compound has been tested and found to be of high quality and purity. 4NP3OBG is also suitable for use in culture media, food testing, and as a fluorogenic substrate.
    Purity:Min. 95%
    Molecular weight:631.54 g/mol

    Ref: 3D-EN04810

    1mg
    305.00€
    2mg
    328.00€
    5mg
    600.00€
  • 4-Nitrophenyl 3-O-(2,3,4,6-tetra-O-acetyl-a-D-mannopyranosyl)-2-O-benzoyl-a-D-mannopyranoside

    CAS:
    4-Nitrophenyl 2,3,4,6-tetra-O-acetyl-b-D-glucopyranoside enzyme substrate is a versatile and reliable reagent for measuring glucosidase activity. It is an ideal substrate for use in assays to detect β-glucosidase activity in a variety of applications, including for in vitro and in vivo studies, and it has been widely used in the pharmaceutical and biotechnology industries. This chromogenic pNP enzyme substrate is designed for effective and precise analysis with good sensitivity and low background signals.
    Purity:Min. 95%
    Molecular weight:735.64 g/mol

    Ref: 3D-EN04784

    5mg
    305.00€
    10mg
    307.00€
    50mg
    599.00€
  • 2-Nitrophenyl 2,3,4-tri-O-acetyl-b-D-galactopyranoside

    CAS:
    2-Nitrophenyl 2,3,4-tri-O-acetyl-b-D-galactopyranoside is a fluorogenic substrate that can be used to detect β-galactosidase activity. This specific substrate is one of the most commonly used substrates for detecting β-galactosidase activity in diagnostic assays. It reacts with the enzyme to produce a blue color and has a fluorescence emission maximum at about 480 nm. The product is available in high purity and high quality and it is also an enzyme substrate.
    Purity:Min. 95%
    Molecular weight:427.36 g/mol

    Ref: 3D-EN04832

    100mg
    305.00€
    500mg
    615.00€
  • Aldol® 457 β-D-galactopyranoside, Biosynth Patent: EP 2427431 and US 8940909

    CAS:
    Aldol 457 Beta-D-galactopyranoside is a chromogenic substrate for beta-galactosidase, which is an enzyme that catalyzes the hydrolysis of the terminal galactose residue in D-galactose to form D-galactitol and D-glucose. Aldol 457 Beta-D-galactopyranoside is used as a reagent for the detection of beta-galactosidase activity in biological samples, such as cultured cells or tissues, and has been shown to be a ligand in binding studies with Galectin 1. This product is also used in diagnostic applications such as food testing, culture media, fluorogenic substrates, environmental testing and more.
    Formula:C24H28N2O8
    Purity:Min. 95.0 Area-%
    Molecular weight:472.49 g/mol

    Ref: 3D-A-4726_P00

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    5g
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    2500mg
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  • 4-Nitrophenyl 3-O-(2,3,4,6-tetra-O-acetyl-a-D-mannopyranosyl)-2-O-benzoyl-4,6-O-benzylidene-a-D-mannopyranoside


    4-Nitrophenyl 3-O-(2,3,4,6-tetra-O-acetyl-a-D-mannopyranosyl)-2-O-benzoyl-4,6-O-benzylidene a -D-mannopyranoside is a synthetic substrate for glycosidases that can be used as an alternative to other substrates. It can be used in the detection of enzyme activity and in the diagnosis of diseases such as diabetes mellitus and Gaucher's disease. 4NP3ODABAB has been shown to have high quality and purity. This substrate has not been evaluated by CAS yet.
    Purity:Min. 95%
    Molecular weight:823.75 g/mol

    Ref: 3D-EN06458

    10mg
    305.00€
    25mg
    330.00€
    50mg
    481.00€
  • Octyl 4-nitrophenyl hexyl phosphonate

    CAS:
    Octyl 4-nitrophenyl hexyl phosphonate is a lipase suicide inhibitor that is a chromogenic substrate for lipases which upon enzymatic action, binds irreversibly to the lipase and inactivates it. The substrate can be used for the determination of active sites in lipases, especially in lipases preferring hydrophobic leaving groups.
    Purity:Min. 95%
    Molecular weight:399.46

    Ref: 3D-EO46652

    10mg
    640.00€
  • 5-Bromo-4-chloro-3-indoxyl nonanoate

    CAS:
    5-Bromo-4-chloro-3-indoxyl nonanoate (5BCNI) is a chromogenic substrate for the detection of alkaline phosphatase. It is soluble in most organic solvents and is stable at room temperature for up to 30 days. 5BCNI has been shown to be a high quality, sensitive and specific substrate for the detection of alkaline phosphatase in culture media, diagnostics, conjugates, environmental testing and chemiluminescence. It has also been used as an enzyme substrate in bioluminescence studies. The CAS number for 5BCNI is 133950-77-3.
    Formula:C17H21BrClNO2
    Molecular weight:386.72 g/mol

    Ref: 3D-B-7410

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  • N-Acetyl-L-serine p-nitroanilide


    N-Acetyl-L-serine p-nitroanilide is a fluorescent probe used for the detection of glucose in urine. It has a high purity and is suitable for use as a substrate for many enzymes, including alkaline phosphatase, β-galactosidase, and peroxidase. N-Acetyl-L-serine p-nitroanilide has been shown to be an excellent indicator of bacterial growth in culture media.

    Formula:C11H12N2O6
    Purity:Min. 95%
    Color and Shape:White powder.
    Molecular weight:268.22 g/mol

    Ref: 3D-EA31320

    1g
    3,725.00€
    2g
    4,470.00€
    5g
    5,587.00€
  • 2-Chloro-4-nitrophenyl 2,3,4,6-tetra-O-acetyl-a-D-glucopyranoside

    CAS:
    2-Chloro-4-nitrophenyl 2,3,4,6-tetra-O-acetyl-a-D-glucopyranoside is a chromogenic substrate used to characterize enzymes in biochemical assays. The substrate is cleaved by enzymes, such as esterases and glycosidases, releasing the chromogenic group, which can be detected by its yellow color.
    Purity:Min. 95%
    Molecular weight:503.84 g/mol

    Ref: 3D-EC06599

    1g
    1,675.00€
    50mg
    305.00€
    100mg
    343.00€
    250mg
    615.00€
    500mg
    1,008.00€
  • 5-Bromo-4-chloro-3-indolyl a-L-arabinopyranoside


    This product is a fluorogenic substrate that reacts with the enzyme beta-galactosidase to produce a fluorescent product. It can be used in food testing, environmental testing, and diagnostics. The CAS number for this product is 609-33-2. This product is available in high purity and high quality.

    Formula:C13H13BrClNO5
    Color and Shape:Powder
    Molecular weight:378.6 g/mol

    Ref: 3D-EB03316

    25mg
    167.00€
    50mg
    225.00€
    100mg
    375.00€
    250mg
    684.00€
    500mg
    1,072.00€
  • 4-Nitrophenyl b-D-cellobioside heptaacetate


    4-Nitrophenyl b-D-cellobioside heptaacetate is a chromogenic substrate for enzymes that catalyze the hydrolysis of glycosidic bonds. This product is used as a diagnostic tool for identifying and characterizing glycosidases and other enzymes that degrade glycosidic bonds. 4-Nitrophenyl b-D-cellobioside heptaacetate is also used in culture media to measure the activity of cellulase enzymes by measuring the release of reducing sugars. The color change of this substrate can be detected at 405 nm, 490 nm, or 550 nm.
    Purity:Min. 95%
    Molecular weight:757.65 g/mol

    Ref: 3D-EN07317

    2mg
    305.00€
    5mg
    328.00€
    10mg
    482.00€
  • 2-Nitrophenyl 2,3,4-tri-O-acetyl-β-D-xylopyranoside

    CAS:
    2-Nitrophenyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is a chromogenic substrate used to detect the enzymatic activity of xylosidase. After cleavage, the nitrophenyl moiety is released exhibiting a light yellowish color. 2-Nitrophenyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside is used in bacterial hemicellulose metabolism assays.
    Formula:C17H19NO10
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:397.33 g/mol

    Ref: 3D-EN04806

    5mg
    258.00€
    10mg
    404.00€
    25mg
    718.00€
  • Aldol® 496 acetate, Biosynth Patent: EP 2427431 and US 8940909

    CAS:
    Aldol 496 acetate is a chromogenic substrate for the enzyme Aldolase. It is also used as a ligand to detect the presence of Aldolase in biological fluids and tissues. This product is designed for Food testing, Diagnostics, Chromogenic substrate, Staining, Enzyme substrate, Bioluminescence, Fluorogenic substrate, Chemiluminescence, and Culture media. Aldol 496 acetate is an organic chemical with high purity and high quality.
    Formula:C27H24N2O4
    Purity:Min. 95.0 Area-%
    Molecular weight:440.49 g/mol

    Ref: 3D-A-4746_P00

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  • N-Acetyl-L-glutamine p-nitroanilide

    CAS:

    N-Acetyl-L-glutamine p-nitroanilide is a fluorogenic substrate that can be used in the diagnosis of bacterial infections. It is a ligand that can bind to enzymes involved in the production of bioluminescence, such as luciferase, and can be used for the detection of bacteria. It is also used for food testing, environmental testing, and diagnostics. N-Acetyl-L-glutamine p-nitroanilide has high purity and high quality, which makes it suitable for conjugation with other molecules.

    Formula:C13H15N3O6
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:309.27 g/mol

    Ref: 3D-EA31322

    1g
    2,980.00€
    2g
    3,725.00€
    5g
    4,842.00€