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Chromogenic Substrates

Chromogenic Substrates

Chromogenic substrates are compounds that produce a visible color change when acted upon by specific enzymes. These substrates are widely used in diagnostic assays, biochemical research, and enzyme-linked immunosorbent assays (ELISAs) to detect and quantify enzyme activity. The color change allows for easy and rapid visual identification of enzyme reactions, making chromogenic substrates essential tools in laboratory analysis. At CymitQuimica, we provide a diverse selection of high-quality chromogenic substrates to support your research and diagnostic applications.

Found 529 products for "Chromogenic Substrates".

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  • 2-Nitrophenyl b-L-fucopyranoside

    CAS:
    2-Nitrophenyl beta-L-fucopyranoside is a chromogenic substrate used to detect the enzymatic activity of beta-L-fucosidase. After cleavage, the nitrophenyl moiety is released exhibiting a light yellowish color. 2-Nitrophenyl beta-L-fucopyranoside is used in bacterial fucose metabolism studies to identify and quantify the enzymatic activity in, for example, Bacteroides spp..
    Purity:Min. 98 Area-%
    Color and Shape:Powder
    Molecular weight:285.25 g/mol

    Ref: 3D-EN07168

    5mg
    239.00€
    10mg
    404.00€
    25mg
    711.00€
    50mg
    1,066.00€
    100mg
    1,776.00€
  • 2-Naphthyl-Beta-D-Glucuronide Sodium Salt

    CAS:
    2-Naphthyl-beta-D-glucuronide sodium salt is an enzyme substrate to detect beta-glucuronidase. The enzyme cleaves the glycosidic bond in 2-naphthyl-beta-D-glucuronide to release 2-naphtol and glucuronic acid. Beta-glucuronidase activity assays are important in many fields such as cancer research, as the enzyme is expressed in some tumors and its activity can be targeted for cancer therapy. The enzyme has also an important role in drug metabolism as it recognizes and binds to drug glucuronide conjugate (as 2-naphthyl-beta-D-glucuronide) facilitating its hydrolysis to release the active part. 2-Naphthyl-beta-D-glucuronide sodium salt has a higher solubility as it is in its salt form.
    Formula:C16H15NaO7
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:342.28 g/mol

    Ref: 3D-EN03259

    5mg
    285.00€
    10mg
    406.00€
    25mg
    713.00€
    50mg
    1,020.00€
    100mg
    1,613.00€
  • 2-Nitrophenyl butyrate

    CAS:

    2-Nitrophenyl butyrate is a chromogenic enzyme substrate that is commonly used in enzymatic assays to detect lipases and esterases. When hydrolyzed by these enzymes, the substrate yields a yellow product that can be detected spectrophotometrically at 405 nm. The advantage of using 2-nitrophenyl butyrate is its high sensitivity and specificity for detecting lipolytic and esterolytic activities. This substrate is widely used in various applications, including enzyme kinetics studies, inhibitor screening, and the determination of esterase activity in biological samples such as serum, plasma, and cell lysates.

    Formula:C10H11NO4
    Purity:Min. 95%
    Color and Shape:Clear Liquid
    Molecular weight:209.2 g/mol

    Ref: 3D-EN15598

    5g
    341.00€
    10g
    455.00€
    25g
    876.00€
  • 2-Naphthyl a-D-glucopyranoside

    CAS:
    2-Naphthyl-lpha-D-glucopyranoside is a substrate for α-glucosidase. 2-Naphthol is released upon hydrolyzation. By simultaneous coupling with a suitable staining reagent, such as hexazonium p-rosaniline, the corresponding reddish-brown azo-dye is formed. Naphthols can also be detected by fluorescence analysis.
    Formula:C16H18O6
    Purity:Min. 98 Area-%
    Color and Shape:Powder
    Molecular weight:306.31 g/mol

    Ref: 3D-EN06601

    500mg
    208.00€
    1g
    363.00€
    2g
    621.00€
    5g
    1,387.00€
    10g
    1,975.00€
  • 5-Bromo-4-chloro-3-indolyl β-D-xylopyranoside

    CAS:

    5-Bromo-4-chloro-3-indolyl b-D-xylopyranoside is an enzyme substrate. This chromogenic substrate is used for beta-D-xylosidase enzyme. In culture media, 5-Bromo-4-chloro-3-indolyl b-D-xylopyranoside is utilised for identification of bacteria such as Klebsiella and Enterobacter.

    Formula:C13H13BrClNO5
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:378.6 g/mol

    Ref: 3D-EB03248

    500mg
    279.00€
    1g
    454.00€
    2g
    838.00€
    5g
    1,920.00€
  • 4-Nitrophenyl 2-acetamido-2-deoxy-3-O-(b-D-galactopyranosyl)-b-D-glucopyranoside

    CAS:
    Chromogenic substrate for fucosyltransferases
    Formula:C20H28N2O13
    Purity:Min. 98 Area-%
    Color and Shape:White Powder
    Molecular weight:504.44 g/mol

    Ref: 3D-EN06439

    1mg
    344.00€
    2mg
    558.00€
    5mg
    1,032.00€
    10mg
    1,459.00€
    25mg
    3,196.00€
  • 2-Nitrophenyl α-D-glucopyranoside

    CAS:

    2-Nitrophenyl a-D-glucopyranoside is a chromogenic substrate commonly used to assay the activity of glucosidases. Upon cleavage of the glycosidic bond by glucosidases, the released 2-nitrophenyl group yields a yellow color detectable by spectrophotometry. This substrate has high sensitivity and is widely used in a variety of applications, including drug discovery and enzyme kinetics studies.

    Formula:C12H15NO8
    Purity:Min. 95%
    Color and Shape:White to off-white solid.
    Molecular weight:301.25 g/mol

    Ref: 3D-EN43851

    50mg
    442.00€
    100mg
    629.00€
    250mg
    968.00€
    500mg
    1,264.00€
    1g
    1,963.00€
  • 4-Nitrophenyl phosphate bis(tris) salt

    CAS:
    Substrate for alkaline phosphatase
    Formula:C6H6NO6P•(C4H11NO3)2
    Purity:(%) Min. 99%
    Color and Shape:Powder
    Molecular weight:461.36 g/mol

    Ref: 3D-EN11786

    50g
    482.00€
    500g
    1,031.00€
    1kg
    1,495.00€
    2kg
    2,755.00€
    5kg
    6,652.00€
  • Aldol® 470 butyrate, Biosynth Patent: EP 2427431 and US 8940909

    Controlled Product
    CAS:
    Aldol® 470 butyrate, a chromogenic substrate manufactured by Cymit Quimica and used for enzymatic assays that produces a yellow-orange color upon enzymatic cleavage.
    Formula:C27H25NO5
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:443.5 g/mol

    Ref: 3D-EA175339

    1g
    500.00€
    2500mg
    968.00€
    5g
    1,419.00€
    10g
    1,975.00€
  • 4-Nitrophenyl laurate

    CAS:
    4-Nitrophenyl laurate is a chromogenic substrate for lipase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl laurate is used in different applications such as the Lipase activity screening, clinical diagnostics
    Formula:C18H27NO4
    Purity:Min. 95%
    Color and Shape:White Powder
    Molecular weight:321.41 g/mol

    Ref: 3D-EN15367

    50g
    337.00€
    100g
    500.00€
    250g
    945.00€
    500g
    1,360.00€
  • 4-Nitrophenyl butyrate

    CAS:
    Chromogenic substrate for esterases
    Formula:C10H11NO4
    Purity:Min. 98%
    Color and Shape:Slightly Yellow Yellow Green Clear Liquid
    Molecular weight:209.2 g/mol

    Ref: 3D-EN15605

    5g
    461.00€
    10g
    691.00€
    25g
    1,025.00€
    50g
    1,442.00€
    100g
    2,409.00€
  • 4-Nitrophenyl sulfate potassium salt

    CAS:
    4-Nitrophenyl sulfate potassium salt is a chromogenic substrate for arylsulfatase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl sulfate potassium salt is used in different applications such as the Metachromatic leukodystrophy diagnosis, sulfatase activity assays
    Formula:C6H4KNO6S
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:257.26 g/mol

    Ref: 3D-EN27113

    1g
    277.00€
    2g
    488.00€
    5g
    911.00€
    10g
    1,494.00€
    25g
    2,343.00€
  • 6-Bromo-2-naphthyl-b-D-xylopyranoside

    CAS:
    6-Bromo-2-naphthyl-b-D-xylopyranoside (BNX) is a chromogenic enzyme substrate used primarily to detect xylosidase activity. It is cleaved by the enzyme, resulting in the release of a colored product that can be measured spectrophotometrically. BNX is widely used as a substrate in research studies for the detection and quantification of various glycoside hydrolases, and it is particularly useful for studying cellulases and xylanases.
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:355.18 g/mol

    Ref: 3D-EB67558

    500mg
    225.00€
    1g
    378.00€
    5g
    980.00€
    10g
    1,443.00€
    25g
    2,811.00€
  • Aldol® 458 nonanoate, Biosynth Patent: EP 2427431 and US 8940909

    Controlled Product
    CAS:

    Aldol 458 nonanoate is a fluorogenic substrate that can be used in the detection of enzyme activity particularly C8/C9-esterases, lipases activity producing a yellow/green-orange color. Aldol 458 nonanoate reacts with enzymes, such as oxidoreductases and hydrolases, to produce light. This product is a conjugate of aldol 458 nonanoate and an enzyme substrate for the detection of these enzymes. It has been shown to be useful in food testing, staining, fluorogenic substrate, environmental testing, chromogenic substrate, conjugate, high purity, enzyme substrate for diagnostics and ligand.

    Formula:C25H29NO4
    Purity:Min. 95%
    Molecular weight:407.5 g/mol

    Ref: 3D-EA175346

    1g
    500.00€
    2500mg
    968.00€
    5g
    1,419.00€
    10g
    1,975.00€
  • Aldol® 484 beta-D-galactopyranoside, Biosynth Patent: EP 2427431 and US 8940909

    CAS:
    Aldol® 484 beta-D-galactopyranoside is a chromogenic enzyme substrate for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid or solid media, yielding intense red color. Aldol® 484 beta-D-galacto-pyranoside can also be used in beta-gal reporter gene assays or for identification of beta-gal positive transformants on agar. Aldol® 484 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.
    Formula:C26H24ClFN2O7
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:530.93 g/mol

    Ref: 3D-EA175350

    250mg
    245.00€
    1g
    500.00€
    2500mg
    946.00€
    5g
    1,338.00€
    10g
    1,784.00€
  • 2-Chloro-4-nitrophenyl-β-D-cellotetraoside

    CAS:
    2-chloro-4-nitrophenyl-beta-D-cellotetraoside is a chromogenic substrate for measuring cellulase activity and other glycosyl hydrolases. The release of 2-chloro-4-nitrophenol can be detected at 405 nm.
    Formula:C30H44ClNO23
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:822.12 g/mol

    Ref: 3D-EC36497

    5mg
    262.00€
    10mg
    417.00€
  • 4-Nitrophenyl 2-acetamido-2-deoxy-a-D-glucopyranoside

    CAS:
    4-Nitrophenyl 2-acetamido-2-deoxy-a-D-glucopyranoside is a chromogenic pNP substrate specifically designed for the analysis of N-acetylglucosaminidase activity. Upon enzyme action, the substrate releases 4-nitrophenol, a yellow compound that can be detected by spectrophotometric methods, providing a reliable and sensitive means of quantifying enzyme activity. This versatile substrate is widely used in biochemical studies, disease diagnostics, and the enzyme production industry.
    Formula:C14H18N2O8
    Color and Shape:White Off-White Powder
    Molecular weight:342.3 g/mol

    Ref: 3D-EN03208

    50mg
    369.00€
    100mg
    570.00€
    250mg
    1,013.00€
    500mg
    1,659.00€
    1g
    2,234.00€
  • 5-Bromo-4-chloro-3-indolyl a-L-arabinofuranoside


    5-Bromo-4-chloro-3-indolyl a-L-arabinofuranoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-L-arabinofuranosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-L-arabinofuranoside is used in bacterial arabinan degradation assays (e.g. Bacillus spp.).
    Formula:C13H13BrClNO5
    Purity:Min. 98.0 Area-%
    Color and Shape:Powder
    Molecular weight:378.6 g/mol

    Ref: 3D-EB09409

    100mg
    325.00€
    250mg
    636.00€
    500mg
    1,056.00€
    1g
    1,942.00€
    2g
    3,491.00€
  • 2-Naphthyl butyrate

    CAS:
    2-Naphthyl butyrate is used as a chromogenic substrate in hematological studies via measuring esterase activity. This type of assays are important as it can help in distinguishing acute leukemias with monocytic or granulocytic differentiation. After hydrolysis of 2-naphthyl butyrate by the esterase (for example acylase), b-napthol is released that can be quantified spectrophotometrically (Exc 320 nm; Em 410 nm).
    Formula:C14H14O2
    Purity:Min. 98 Area-%
    Color and Shape:Colorless Clear Liquid
    Molecular weight:214.26 g/mol

    Ref: 3D-EN10875

    250mg
    261.00€
    500mg
    356.00€
    1g
    497.00€
  • 6-Bromo-2-naphthyl α-D-mannopyranoside

    CAS:
    Chromogenic substrate to visualize the activity of alpha-D-mannopyranoside; red color produced
    Formula:C16H17BrO6
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:385.21 g/mol

    Ref: 3D-EB05122

    100mg
    185.00€
    250mg
    283.00€
    500mg
    437.00€
    1g
    526.00€