Métabolisme

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig HDL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig HDL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig HDL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig HDL in the samples is then determined by comparing the OD of the samples to the standard curve.

hCAII/XII-IN-1 (compound 4l) is a potent inhibitor of hCAXII and hCAII, with Ki values of 8.4 nM and 9.4 nM, respectively. It plays a significant role in cancer research.

11R-CaN-AID is a potent, cell-permeant inhibitor of calcineurin [1] [2].

UG-480 is a Gemini analog that effectively stabilizes the active VDR conformation. It exhibits antiproliferative effects in estrogen receptor-positive MCF-7 breast adenocarcinoma cells and is utilized in cancer research.

LH1513 is a dioxalate salt derivative of l-lysine that inhibits CaOx crystallization more effectively than citrate and pyruvate. It shows potential preventive activity in hyperoxaluria models and effectively prevents urinary CaOx crystal formation in Agxt knockout mice. AGXT-1 is a mitochondrial protein involved in metabolic processes.

3,4-Dihydroxyamphetamine (α-Methyldopamine) (hydrochloride) is a minor metabolite of 3,4-Methylenedioxymethamphetamine (MDMA) and exhibits cytotoxicity in rat hepatocytes. Additionally, 3,4-Dihydroxyamphetamine (hydrochloride) is applicable in hypertension research.

H-Met-Val-OH is a dipeptide inhibiting FMO1 and FMO3 and promoting neurite outgrowth, relevant for neuroregenerative and enzymatic modulation studies.

13,14-dehydro-15-cyclohexyl Carbaprostacyclin, a stable PGI2 analog, impedes ADP-induced platelet aggregation; ED50 ~40 nM in PRP, 77 nM in washed platelets.

YYLLVR is an inhibitory peptide of angiotensin-converting enzyme (ACE) with an inhibition rate of 89.10%. It demonstrates a low binding energy to ACE at -35.98 kcal/mol. YYLLVR can be utilized in hypertension research.

Resinacein L is an inhibitor of α-glucosidase with an IC50 of 0.635 mM. This compound can slow the digestion and absorption of carbohydrates, thereby reducing postprandial blood glucose levels.

13-cis-Retinyl acetate: active 13-cis isomer of Retinyl acetate, metabolite of vitamin A.

Bifenazate-diazene, a significant degradation product of Bifenazate, is characterized as a selective carbazate acaricide and insecticide [1].

DPTIP hydrochloride is a potent inhibitor of neutral sphingomyelinase (N-SMase 2) that can cross the blood-brain barrier. It acts as an exosome inhibitor with an IC50 value of 30 nM.

Axl-IN-16 (Compound 4), an Axl inhibitor, not only suppresses Axl expression and HIF activity but also triggers fruiting body formation in Flammulina velutipes.

α-Glycerophosphate Dehydrogenase-Triosephosphate (GDH-TIM) is an enzyme mixture composed of glycerophosphate dehydrogenase (GDH) and triosephosphate isomerase (TIM). This compound is employed to measure transketolase (TK) activity in erythrocyte hemolysates, which helps in assessing vitamin B deficiency.

α-Amylase/α-Glucosidase-IN-14 is an orally bioactive inhibitor of α-amylase and α-glucosidase, with IC50 values of 45.53 μM and 27.73 μM, respectively.

α-Amylase/α-Glucosidase-IN-9 (compound 5h) is a dual inhibitor of α-amylase (IC50= 16.4 μM) and α-glucosidase (IC50= 31.6 μM).

α-Amylase/α-Glucosidase-IN-15 (compound 6C) is an orally active inhibitor of α-glucosidase (α-Glucosidase) and α-amylase (α-amylase), with IC50 values of 21 μM and 61 μM, respectively.