Fluorogenic Substrates

<p>Fluorogenic substrate for fucosidase</p>

<p>Fluorogenic substrate for ?-arabinofuranosidase</p>

<p>6-Chloro-4-(trifluoromethyl)umbelliferyl b-D-cellotrioside is a fluorogenic substrate for cellulases and beta-glucosidases. After hydrolisis, the fluorophore TFMU is released generating a blue fluorescence.</p>

<p>Lipase targeting MOSCERDAM® substrate</p>

<p>Fluorogenic cellulose analogue</p>

<p>The 4-Methylumbelliferyl b-D-ribofuranoside is a top-notch fluorogenic substrate for glycosidase activity detection. Upon hydrolysis by enzymes, it generates a strongly fluorescent 4-methylumbelliferone molecule, enabling rapid assay results and real-time monitoring of enzymatic reactions. This product is suitable for many biological research applications, such as in vitro analysis, diagnostic testing, and drug discovery. With its high sensitivity and specificity, it delivers consistent and accurate results, making it an essential tool for scientists and researchers.</p>

<p>Fluorogenic substrate for chitinase</p>

<p>A sensitive fluorogenic substrate for fluorometric and UV-spectrophotometric assays of phosphatases. It is used for the detection of C. perfringens because acid phosphatase is a highly specific indicator for C. perfringens.</p>

<p>4-Methylumbelliferyl N,N',N''-triacetyl-beta-D-chitotrioside is a fluorogenic substrate for chitinase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl N,N',N''-triacetyl-beta-D-chitotriosideas a substrate for measuring the chitinase activity is used for fungal infection assays.</p>

<p>4-Trifluoromethylumbelliferyl β-D-galactopyranoside is a fluorogenic substrate for beta-galactosidase&Tab;. Upon hydrolysis, the fluorogenic moiety (blueish) 4-trifluoromethylumbelliferone (also known as TFMU) is released. 4-Trifluoromethylumbelliferyl β-D-galactopyranoside is commonly used in bacterial detection and in LacZ gene expression assays.</p>

<p>4-Methylumbelliferyl 6-deoxy-2,3,4,6-tetra-O-acetyl-6-thio-beta-D-glucopyranoside is a fluorogenic substrate for beta-glucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 6-deoxy-2,3,4,6-tetra-O-acetyl-6-thio-beta-D-glucopyranosideas a substrate for measuring the beta-glucosidase activity is used for Gaucher disease diagnosis.</p>

<p>Glutaryl-glycyl-L-arginine 7-amido-4-methylcoumarin hydrochloride is a synthetic peptide substrate used as a fluorogenic substrate for aminopeptidase.</p>

<p>Fluorogenic substrate for esterase with C8 activity yielding a blue fluorescent solution upon cleavage. Used for the identification of Salmonella spp. based on the detection of caprylate esterase.</p>

<p>Fluorogenic substrate for carboxylesterase</p>

<p>4-Methylumbelliferyl nonanoate is a synthetic fluorogenic substrate for lipase. It is used to measure the activity of lipases in vitro. 4-Methylumbelliferyl nonanoate reacts with a lipid substrate, such as sodium taurocholate, to produce 4-methylumbelliferone and dodecanol. The fluorescent product can be detected by fluorimetric or spectrophotometric methods. This compound is activated by an enzyme preparation, such as proteolytic enzymes, and can be used to measure the activity of these enzymes in vitro.</p>

<p>Fluorogenic substrate for beta-glucuronidase GUS</p>

<p>Methylumbelliferyl butyrate is structurally based on 4-methylumbelliferone and is a MOSCERDAM® substrate. It targets the esterase enzyme.</p>

<p>4-Methylumbelliferyl beta-D-cellohexaoside is a fluorogenic substrate for cellulase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl beta-D-cellohexaosideas a substrate for measuring the cellulase activity is used for detecting bacterial infection and gut microbiome analysis.</p>

<p>4-Methylumbelliferyl 2-deoxy-2-sulfamino-a-D-glucopyranoside sodium salt (M3A or MU-GlcNS) is a fluorogenic substrate of heparin sulfamidase, used in the diagnosis of Mucopolysaccharidosis III type A (MPS III A). MPS III A, also known as Sanfilippo syndrome type A, is caused by the deficiency of heparan N-sulfatase. Heparin sulfamidase in the assay desulfates MU-GlcNS to 4-methylumbelliferyl 2-amino-2-deoxy-α-D-glucopyranoside (MU-GlcNH2). To release fluorometrically measurable 4-methylumbelliferone (MU) and by-product glucosamine, MU-GlcNH2 is further hydrolysed by an enzyme such as yeast α-glucosidase during a second incubation.</p>

<p>Fluorogenic substrate for carboxylesterases</p>