Fluorogenic Substrates

<p>4-Methylumbelliferyl elaidate is a fluorogenic substrate for lipase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl elaidateas a substrate for measuring the lipase activity is used for assessment of lipase activity to identify pancreatitis.</p>

<p>4-Methylumbelliferyl alpha-D-mannopyranoside (4-MU-a-D-Man) is a fluorogenic substrate used in activity assays for alpha-mannosidase. When it is hydrolyzed by alpha-mannosidase, it produces a fluorescent compound, 4-methylumbelliferone (4-MU). 4-MU-a-D-Man is often used in medical research to monitor the activity of alpha-mannosidase, which is important in lysosomal storage diseases.  In addition, 4-Methylumbelliferyl alpha-D-mannopyranoside has been used to study the the activity of concanavalin A, a lectin that specifically binds to alpha-D-mannopyranosyl and alpha-D-glucopyranosyl residues on various glycoproteins. The use of this fluorogenic enzyme substrate permits study the degree of alpha-D-mannosidic linkage cleavage by the enzyme.</p>

<p>Fluorogenic substrate for peroxidase</p>

<p>Resorufin b-D-glucopyranoside is a stable red fluorogenic galactosidase substrate.</p>

<p>4-Methylumbelliferyl-alpha-L-iduronic acid, cyclohexylammonium salt is a fluorogenic substrate for the detection of alpha-L-iduronidase activity. It has been used to measure the activity of this enzyme in cells and tissues and as an indicator of tissue damage. The product is also used in food testing, diagnostics, and environmental testing.</p>

<p>Fluorogenic substrate for N-acetyl-β-D-galactosaminidase. Detection of Candida albicans.</p>

<p>Methylumbelliferyl oleate is a MOSCERDAM® substrate targeting the lipase enzyme</p>

<p>Resorufin-beta-D-glucuronic acid, sodium salt is a fluorogenic substrate that can be used in diagnostic applications. It is a conjugate of resorufin and beta-D-glucuronic acid. The enzyme substrate reacts with the enzyme luciferase to produce light. The high purity and quality of this product make it ideal for use in the detection of the presence of glucose in urine, which can be used to diagnose diabetes mellitus.</p>

<p>4-Methylumbelliferyl-beta-D-ribofuranoside is a chemical compound that belongs to the class of phenols. It has been found in Australian plants and animals. 4-Methylumbelliferyl-beta-D-ribofuranoside is used as a corrosion inhibitor. This chemical has been shown to be effective against corrosion in mammals.br&gt;br&gt;<br>4-Methylumbelliferyl-beta-D-ribofuranoside can also be used as a fluorescent probe for studying cell membrane activity.</p>

<p>4-Methylumbelliferyl lignocerate is a fluorogenic substrate for lipase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl lignocerateas a substrate for measuring the lipase activity is used for X-linked adrenoleukodystrophy diagnosis and peroxisomal disorder screening.</p>

<p>4-Methylumbelliferyl-beta-D-fucopyranoside is a fluorogenic substrate for beta-fucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl-beta-D-fucopyranosideas a substrate for measuring the beta-fucosidase activity is used for the diagnosis of fucosidosis and the study of glycoprotein metabolism disorder.</p>

<p>The DDAO molecule [7-hydroxy-9H-(1, 3-dichloro-9, 9 dimethylacridin-2-one] is used as a stain and a dye in molecular biology, due to its ability to bind intracellular and extracellular DNA. DDAO galactoside is a fluorogenic substrate for galactosidase.</p>

<p>A substrate used for the diagnosis of Sanfilippo disease C (MPS III C).</p>

<p>Living cells of Escherichia coli can be reliably detected with media that contain a chromogenic or fluorogenic substrate for beta-glucuronidase (also termed β-D-glucuronidase), an enzyme that occurs almost exclusively in E. coli. RUG®, Resorufin-beta-D-glucuronic acid methyl ester is a highly sensitive chromogenic and fluorogenic indicator for E. coli. After removal of the methyl ester group by unspecific hydrolysis, orange colored product is cleaved by beta-glucuronidase in liquid media or on agar plates and yields an intense pink color. Red fluorescence is obtained upon excitation at 560-570 nm.Formally ER175455.</p>

<p>Sensitive fluorogenic substrate for fluorometric and UV-spectrophotometric assays of phosphatases. This may also be used for in-gel assays</p>

<p>PAIRED PRODUCT AVAILABLE: alpha-L-iduronidase Enzyme, FI1793834MU-alpha-L-iduronide-2-sulphate (M2) is a fluorogenic substrate of iduronate-2-sulphatase (IDS), used in a 2-step assay coupled with alpha-L-iduronidase. The assay is used in biochemical testing for Hunter syndrome (mucopolysaccharidosis II / MPS II). In a first step, IDS from biological samples cleaves the sulphate group from M2. In a second step, exogeneous alpha-L-iduronidase which cleaves the 4-MU moiety is added to the reaction, resulting in release of a fluorescent signal (Ex 360 nm / Em 455 nm). The reaction is stopped by the addition of sodium carbonate.</p>

<p>6-Hexadecanoylamino-4-methylumbelliferyl phosphorylcholine (HMUPC) is a fluorogenic substrate of acid sphingomyelinase (ASM) in the application of the diagnosis of Niemann–Pick type A and B (NPAB). NPAB is one of the lysosomal storage diseases, caused by a deficiency of ASM. This deficiency results in the accumulation of sphingomyelin and other lipids in various tissues. The assay using HMUPC as the substrate shows rapid and robust determination of ASM activity. When combined with the unlabelled natural substrate such as sphingomyelin as a specific inhibitor of ASM activity, the specificity of assay is enhanced up to the level of the classical assay with radiolabelled natural substrate. HMUPC is also used as the substrate in the ASM assay to investigate the retention of lysosomal enzyme activity of bi-functional IgG-ASM fusion protein in the delivery across blood-brain barrier.</p>

<p>4-Methylumbelliferyl-beta-D-glucuronic acid methyl ester is a chromogenic substrate for β-glucuronidase. It is used in diagnostic applications and as a substrate in enzyme kinetics experiments. 4-Methylumbelliferyl-beta-D-glucuronic acid methyl ester has fluorescence properties that allow it to be detected by chemiluminescence and bioluminescence.</p>

<p>Fluorogenic substrate for carboxylesterases</p>

<p>Fluorogenic substrate for proglutamyl peptidase 1 yielding a blue fluorescent solution upon cleavage. Used in identification of Enterococcus spp. and group A streptococci (Streptococcus pyogenes).</p>