Fluorogenic Substrates
In this category, you will find a comprehensive selection of fluorogenic substrates designed for enzymatic catalysis. These substrates are used with various types of enzymes and are capable of producing color and fluorescence upon reaction. Fluorogenic substrates are essential tools in biochemical and molecular biology research, enabling the detection and quantification of enzyme activity with high sensitivity and specificity. They are widely used in assays for studying enzyme kinetics, screening for enzyme inhibitors, and monitoring cellular processes. At CymitQuimica, we provide high-quality fluorogenic substrates to support your research, ensuring reliable and accurate results in your enzymatic assays.
Found 284 products for "Fluorogenic Substrates".
Sort by
Purity (%)
0
100
|
0
|
50
|
90
|
95
|
100
Resorufin N-acetyl-b-D-glucosamine
CAS:Resorufin N-acetyl-b-D-glucosamine is fluorogenic substrate for N-acetyl-beta-D-glucosaminidase (NAG).
Formula:C20H20N2O8Purity:Min. 98 Area-%Color and Shape:Orange PowderMolecular weight:416.38 g/mol4-Methylumbelliferyl 6-thio-palmitate-b-D-glucopyranoside - Moscerdam™ biochemical purity
CAS:4-Methylumbelliferyl 6-thio-palmitate-b-D-glucopyranoside (Mu-6S-Palm-β-Glc) is a fluorogenic substrate of palmitoyl-protein thioesterase 1 (PPT1), used in the diagnosis of infantile neuronal ceroid lipofuscinosis (INCL). INCL, also known as infantile CLN1 disease, is a rapidly progressing lysosomal storage disorder (LSD), caused by a deficiency of PPT1. Mu-6S-Palm-β-Glc is also used as the substrate in the PPT assay to investigate the retention of lysosomal enzyme activity of bi-functional IgG-PPT1 fusion protein in the delivery across blood-brain barrier.
Formula:C32H48O8SPurity:Min. 95%Color and Shape:White PowderMolecular weight:592.78 g/molL-Leucine 7-amido-4-methylcoumarin hydrochloride salt
CAS:L-Leucine 7-amido-4-methylcoumarin hydrochloride salt is a synthetic peptide substrate used as a fluorogenic substrate for leucine aminopeptidase.Formula:C16H21ClN2O3Purity:Min. 98 Area-%Color and Shape:White PowderMolecular weight:324.8 g/mol4-Methylumbelliferyl caprylate
CAS:Used for identification of Salmonella spp. by detection of caprylate esteraseFormula:C18H22O4Purity:Min. 95%Color and Shape:White PowderMolecular weight:302.36 g/molEthyl umbelliferone-3-carboxylate-α-D-glucopyranoside
Ethyl umbelliferone-3-carboxylate-a-D-glucopyranoside is a fluorogenic substrate used for detecting alpha-glucosidase activity in enzymatic assays. When alpha-glucosidase cleaves , the glycosidic bond, umbelliferone-3-carboxylate is released, which exhibits strong fluorescence. This substrate is commonly utilized in glycobiology research, diagnostic tests, and high-throughput screening of enzyme inhibitors.
Formula:C18H20O10Purity:Min. 95%Color and Shape:Off-white to yellow solid.Molecular weight:396.35 g/molL-Alanine-7-amido-4-methylcoumarin hydrochloride
CAS:L-Alanine-7-amido-4-methylcoumarin hydrochloride is a synthetic peptide substrate used as a fluorogenic substrate for alanine aminopeptidase.Formula:C13H14N2O3·HClPurity:Min. 90%Color and Shape:PowderMolecular weight:282.72 g/mol4-Methylumbelliferyl 2-acetamido-2-deoxy-a-D-glucopyranoside
CAS:4-Methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside is a fluorogenic substrate for alpha-N-acetylglucosaminidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranosideas a substrate for measuring the alpha-N-acetylglucosaminidase activity is used for Sanfilippo syndrome B screening and mucopolysaccharidosis diagnosis.Formula:C18H21NO8Purity:Min. 98 Area-%Color and Shape:PowderMolecular weight:379.36 g/mol4-Methylumbelliferyl-N-acetyl-β-D-glucosaminide-6-sulfate, sodium salt
CAS:4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt is a high-quality fluorogenic substrate specifically designed for the detection and quantification of various enzymes, including beta-glucosidases and sulfatases. Upon hydrolysis by the target enzyme, this substrate releases the highly fluorescent 4-methylumbelliferone, allowing for real-time monitoring and accurate measurement of enzyme activities in biochemical assays, diagnostic tests, and research applications. 4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt has excellent solubility in water, ensuring a consistent and reliable performance in different experimental conditions. This versatile substrate is an essential tool for researchers and industry professionals who require a sensitive and reproducible detection method for enzymes of interest.Formula:C18H20NNaO11SPurity:Min. 98.0 Area-%Molecular weight:481.41 g/molRef: 3D-M-5505
-Unit-ggTo inquire5mgTo inquire25mgTo inquire50mgTo inquire100mgTo inquire250mgTo inquire4-Methylumbelliferyl 2-deoxy-2-sulfamino-b-D-glucopyranoside sodium salt
4-Methylumbelliferyl 2-deoxy-2-sulfamino-beta-D-glucopyranoside sodium salt is a fluorogenic substrate for N-sulfoglucosamine sulfohydrolase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-deoxy-2-sulfamino-beta-D-glucopyranoside sodium saltas a substrate for measuring the N-sulfoglucosamine sulfohydrolase activity is used for screening of Mucopolysaccharidosis IIIA (MPS IIIA) and diagnosis of Sanfilippo A syndrome.Formula:C16H19NO10SNaPurity:Min. 95%Color and Shape:PowderMolecular weight:439.37 g/mol3-(4,4-Difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionamido)propyl b-D-lactoside
3-(4,4-Difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionamido)propyl b -D -lactoside (FDPBP) is a substrate for alkaline phosphatase. It reacts with the enzyme to produce a blue color. FDPBP is used in diagnostic applications such as food testing and bioluminescence.Purity:Min. 95%Color and Shape:PowderMolecular weight:675.48 g/mol4-Methylumbelliferyl 2-acetamido-2-deoxy-α-D-glucopyranoside-6-sulfate potassium
CAS:4-Methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside-6-sulfate potassium is a fluorogenic substrate for N-acetylglucosamine-6-sulfatase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside-6-sulfate potassiumas a substrate for measuring the N-acetylglucosamine-6-sulfatase activity is used for screening of Mucopolysaccharidosis IIID (MPS IIID) (a rare lysosomal storage disorder) and Sanfilippo D syndrome diagnosis.Formula:C18H20KNO11SPurity:Min. 95%Color and Shape:White PowderMolecular weight:497.52 g/molFLsharp™ - Phosphate, disodium salt
FLsharp™ - Phosphate, disodium salt is a fluorogenic water-soluble substrate for phosphatase enzymes. Its chemical name is 6-Chloro-2-[5-chloro-2-(phosphonooxy)phenyl]-4(1H)-quinazolinone disodium salt and was developed by Molecular Probes in the 1990s. After cleavage of the substrate by phosphatase activity, an insoluble product is formed that is highly fluorescent in the solid state. Upon UV irradiation this very stable fluorophore emits green light in the 530 nm range for an extended period of time. FLsharp™ is a unique substrate - optimized for the detection of phosphatase activity in solid matrices such as agar media, tissue sections or microscopic preparations. FLsharp™ - Phosphate, disodium salt is used in microbiology as a substrate for bacterial phosphatases.
Purity:Min. 95%Fluorescein diacetate
CAS:Fluorogenic substrate targeting esterases , hGSTP1-1
Formula:C24H16O7Color and Shape:PowderMolecular weight:416.38 g/mol4-Methylumbelliferyl β-D-glucuronide
CAS:Fluorogenic substrate for b-glucuronidase GUSFormula:C16H16O9Purity:Min. 95%Molecular weight:352.29 g/molFluorescein di-beta-D-galactopyranoside
CAS:Fluorogenic substrate for beta-galactosidase
Formula:C32H32O15Purity:Min. 98 Area-%Color and Shape:Slightly Yellow PowderMolecular weight:656.59 g/mol4-Trifluoromethylumbelliferyl b-D-glucuronide potassium salt
CAS:4-Trifluoromethylumbelliferyl b-D-glucuronide potassium salt is a fluorogenic substrate for beta-glucuronidase. Upon hydrolysis, the fluorogenic moiety (blueish) 4-trifluoromethylumbelliferone (also known as TFMU) is released. 4-Trifluoromethylumbelliferyl b-D-glucuronide potassium salt is commonly used in bacterial detection and for drug metabolism studies as well as lysosomal storage disorder screening.Formula:C16H12F3O9KPurity:Min. 95 Area-%Color and Shape:White PowderMolecular weight:444.35 g/mol4-Methylumbelliferyl sulfate potassium salt
CAS:4-Methylumbelliferyl sulfate potassium salt serves as a superior fluorogenic substrate in enzyme detection assays. Upon enzymatic hydrolysis, it releases a highly fluorescent 4-methylumbelliferone moiety, enabling real-time analysis and quantification of enzyme activities. This product is ideal for a variety of biological assay applications, including in vitro diagnostics, drug discovery, and research purposes. Its excellent sensitivity, specificity, and performance ensure accurate and consistent results every time.Formula:C10H7KO6SPurity:Min. 98 Area-%Color and Shape:White PowderMolecular weight:294.32 g/mol4-Methylumbelliferyl-alpha-L-rhamnopyranoside
CAS:4-Methylumbelliferyl-alpha-L-rhamnopyranoside is a fluorogenic substrate for alpha-L-rhamnosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl-alpha-L-rhamnopyranosideas a substrate for measuring the alpha-L-rhamnosidase activity is used for detection of bacterial infection.Formula:C16H18O7Purity:Min. 98 Area-%Color and Shape:PowderMolecular weight:322.32 g/mol6'-(Diethylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl b-D-glucopyranoside
6'-(Diethylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl b-D-glucopyranoside is a fluorogenic substrate that is used in diagnostic applications. It has been shown to be a useful ligand for the determination of enzyme activity, and it can also be used to detect bacterial culture media contaminants. 6'-(Diethylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl b-D-glucopyranoside is used as an enzyme substrate in chemiluminescent assays, and with other reagents, it can be used for the detection of environmental contaminants. This product has a CAS number of 55426-02-8 and can be found on the website www.sigmaaldrich.com/catalog/productPurity:Min. 95%Molecular weight:494.49 g/mol4-Methylumbelliferyl 4-O-(α-L-fucopyranosyl)-β-D-galactopyranoside
CAS:4-Methylumbelliferyl 4-O-(alpha-L-fucopyranosyl)-beta-D-galactopyranoside is a fluorogenic substrate for alpha-L-fucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 4-O-(alpha-L-fucopyranosyl)-beta-D-galactopyranosideas a substrate for measuring the alpha-L-fucosidase activity is used for fucosidosis diagnosis and in screening studies of glycoprotein metabolism disorders.Formula:C22H28O12Purity:Min. 95 Area-%Color and Shape:PowderMolecular weight:484.45 g/mol
![6'-(Diethylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl b-D-glucopyranoside](/_next/image/?url=https%3A%2Fstatic.cymitquimica.com%2Fproducts%2F3D%2Fthumb-webp%2FED139090.webp&w=3840&q=75)
