DNA Damage/DNA Repair

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PARP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PARP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PARP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PARP2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Fleroxacin (RO 23-6240) is a broad-spectrum antimicrobial fluoroquinolone. It strongly inhibits the DNA-supercoiling activity of DNA gyrase.

Tallimustine HCl, an AT-specific alkylating agent, treats leukemia in immunodeficiency mouse models.

Mavodelpar free acid hydrochloride (Pparδ agonist HCl) is a potent PPARδ agonist.

Ciprofloxacin monohydrochloride (Bay-09867 hydrochloride) is a broad-spectrum antimicrobial carboxyfluoroquinoline.

LTURM34 is an inhibitor of DNA-PK with IC50 of 34 nM.

5-Azacytidine (Ladakamycin) is a cytidine nucleoside analog, a DNA methylation inhibitor with specificity.

Balofloxacin, a quinolone antibiotic, can inhibit the synthesis of bacterial DNA by interfering with the DNA gyrase.

Cisplatin (CDDP) is a DNA cross-linking agent.

Methotrexate (WR19039) is a folate analog, an inhibitor of the dihydrofolate reductase DHFR.

G150 suppresses DSDNA-induced interferon; IC50: 10.2 nM. It's a potent, selective H-CGAS inhibitor.

Telomerase-IN-2 (Telomerase inhibitor 2) shows potent inhibitory activity against telomerase by decreasing the expression of dyskerin (IC50: 0.89 µM).

Foscarnet sodium (Phosphonoformate) is an antiviral agent used in the treatment of cytomegalovirus retinitis.

Levetiracetam (SIB-S1) is a relatively unique anticonvulsant that is typically used in combination with other antiepileptic medications for partial onset

STL127705 inhibits Ku 70/80 protein & DNA-PKCS kinase, IC50s: 3.5 μM & 2.5 μM.

Mifobate (SR-202) 是一种有效且特异性的 PPARγ拮抗剂，可选择性抑制噻唑烷二酮 (TZD) 诱导的 PPARγ 转录活性，IC50为140 μM，具有抗肥胖和抗糖尿病作用。

Cloxiquine (Dermofongin) is a monohalogenated 8-hydroxyquinoline with activity against bacteria, fungi, and protozoa.

Indomethacin sodium hydrate (Indometacin sodium hydrate) is a COX1/2 inhibitor that induces migraine headaches and may be used in studies of rheumatoid joints.

Altretamine (ENT-50852) is an alkylating agent with antineoplastic activity.

Chlorambucil (CB-1348) is an orally-active antineoplastic aromatic nitrogen mustard.